Currently, there is a lack of techniques for rapid on-site screening of body fluids, such as blood, fingerprints, and sweat. Each body fluid contains a specific set of clinical biomarkers that can be exploited for the determination of originator properties via point-of-care (POC) diagnostic approaches. These metabolites can be detected in various combinations to allow for a range of applications from the identification of an individual to the diagnosis of a disease. This concept has successfully been used for the determination of age from blood, of biological sex from fingerprints, and of individual differentiation from sweat. Biomedical diagnostic systems capable of detecting and monitoring metabolites related to an individual’s state-of-health facilitate a diagnosis and treatment by less-skilled medical professionals. Our research requires the identification of new metabolic markers for the development of rapid and non-invasive methods with forensic, clinical, and security applications.